Scientists at Rejuveron Vascular Therapeutics AG, a company specializing in the development of therapies to tackle neurodegeneration, are using INTEGRA’s VIAFLO 384 handheld electronic pipette to accelerate their research. Combining this convenient benchtop system with endothelial cells cultured in Corning® HTS Transwell® 96 well plates allows the team to investigate the ability of drugs to penetrate the blood brain barrier (BBB).
Image Credit: INTEGRA
Vasco Ledebur de Antas de Campos, Head of Discovery Biology at Rejuveron Vascular Therapeutics, explained: “We deposit cells in the Transwells, and incubate them to form a monolayer on the filter membrane in each well. Once we have obtained high confluency – which simulates the BBB – we administer the drug candidates.”
“We use a VIAFLO 384 – with a 96 channel head and 125 µl Sterile Filter GripTips – to both deliver the drugs to the apical side and take samples from the basolateral side of the Transwells. This allows us to check the amount of each drug that was able to permeate the barrier. This approach would be difficult to perform with a standard multichannel pipette, as we are often screening large libraries. In contrast, it’s a simple task for the VIAFLO 384, since you can easily program the system to aspirate from any part of the well.”
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INTEGRA Biosciences (https://www.integra-biosciences.com) is a leading provider of high-quality laboratory tools and consumables for liquid handling and media preparation. The company is committed to creating innovative solutions which fulfil the needs of its customers in research, diagnostics and quality control within the life sciences markets and medical sector. Today, INTEGRA innovative laboratory products are widely used all around the world.
More than 100 distribution partners form a worldwide sales network providing responsive and competent services to customers. These distribution partners are supported by a highly motivated and experienced team of specialists at the company headquarters in Zizers, Switzerland and Hudson, NH, USA. INTEGRA is an ISO 9001 certified company.